Karyotype Lab

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Prelab Worksheet

(Write the questions and answers in your lab notebooks.)
  1. Imagine a collection of socks of different sizes, colors, and patterns. Did you picture in your mind at least 23 pairs? Separate the socks and mix them up in the middle of the floor. Now reunite the members of each pair and arrange the pairs from large to small. If you were asked to locate the small hole in the pair of worn blue socks, how would you describe its location? Create a system for identifying a specific location on these socks considered as a group. It may be easiest to draw your answer.
  2. Below is a picture of a metaphase spread. Metaphase is a stage in the cell division cycle prior to division. Do the small dark objects resemble a bunch of miniature socks scattered on the floor, or do they resemble miniature buildings full of individual apartments? Think of both analogies and create an address system for these structures, called chromosomes, in which a specific location can be identified.

 

Lab Worksheet

MATERIALS PROCEDURE  

HUMAN CHROMOSOMES

METAPHASE SPREAD SHEET: MALE

 

HUMAN CHROMOSOMES

METAPHASE SPREAD SHEET: MALE

Human Karyotype Form

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1
2
3
4
5
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6
7
8
9
10
11
12
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13
14
15
16
17
18
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19
20
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22
X
Y

Postlab Worksheet

You have prepared a karyotype from a picture of human chromosomes called a metaphase spread. Look carefully at your karyotype, and recall the metaphase spread as you answer the following questions.
  1. Summarize the relationship between the size of the chromosome and the number assigned to it.
  2. What are the exceptions to the pattern noted in question 1?
  3. In what ways are the female and male karyotypes alike, and in what ways are they different? You may need to look at a classmate's karyotype.
  4. Describe the general structure of a chromosome and the kinds of structural variation that exist among different chromosomes.
  5. Describe the patterns of light and dark areas (banding) found on the chromosomes.
  6. What advantage might chromosomal banding patterns have in the analysis and diagnosis of chromosomal problems or abnormalities